Saroj Velamakanni
Fahy Gurteen Laboratories, UK
Title: Multiparameter analysis using cell cycle biomarkers for breast cancer: Prognostic and predictive implications
Biography
Biography: Saroj Velamakanni
Abstract
Cell cycle related molecules play a pivotal role in maintaining genomic stability and can be used as biomarkers for cell cycle phase distribution. In this study, we performed immunohistochemical multiparameter analysis using five cell cycle related biomarkers for a cohort of 182 patients and linked it with clinicopathological information. Various stages involved in the design, development and validation of proprietary antibodies against these biomarkers will be discussed. Three unique cell cycle phenotypes were identified 1) out-of-cycle state 2) G1 delayed/arrested state and 3) accelerated cell cycle progression state. Our algorithm sheds new light into the cell cycle state of dynamic tumour populations in vivo. The information obtained is of major prognostic significance and may impact on individualised therapeutic decisions.
Speaker Presentations
Speaker PDFs
Speaker PPTs Click Here