David Meininger
TRIANNI, USA
Title: The Trianni Mouse — the next generation transgenic platform for the isolation of fully human monoclonal antibodies
Biography
Biography: David Meininger
Abstract
Trianni offers a fully humanized monoclonal antibody platform. The Trianni’s technology is distinguished by key advantages of ease of use, comprehensive antibody diversity and in vivo selection. Founded in 2010, The Trianni Mouse is an antibody discovery platform with several distinctive and highly attractive features. Chief among these is the fact that it expresses a full repertoire of variable domains for human antibody heavy and light chains. In generating its platform, Trianni employed a unique combination of in silico gene design, DNA synthesis and targeted genomic mutagenesis to modify all three mouse antibody chain loci (heavy, kappa and lambda). The new gene segments embedded in these loci have a novel proprietary optimized structure involving human coding sequences (of V, D and J) and mouse regulatory sequences (promoters, enhancers, introns, recombination signal sequences). This feature distinguishes the Trianni Mouse from all other similar platforms now available. The platform has been extensively validated both internally and externally (adopted by several major pharma companies) and has proven itself as a powerful resource for the isolation of high quality therapeutic monoclonal antibodies, i.e., effective in terms of hybridoma frequency, antibody titers, affinities and biological effector function. Trianni continues to enhance and expand the platform, generating mice that respond to evolutionary conserved sequences; respond to antigens with non-immunoglobulin scaffolds and produce bispecific antibodies.
